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KMID : 0380020190340030154
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Korean Journal of Biotechnology and Bioengineering
2019 Volume.34 No. 3 p.154 ~ p.158
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Study of Anti-Obesity Effect of Rumex Japonicus Houttuyn Ethanol Extracts in 3T3-L1 Preadipocytes cells
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Nam Gun-He
Jo Kyung-Jo
Park Ye-Seul
Kawk Hye-Won
Wee Ji-Hyang
Kim Sang-Yung
Koo Bong-Seong
Kim Young-Min
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Abstract
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Rumex japonicus Houttuyn is known for its antiinflammatory and anti-microbial effects. In this study, we determined the effects of ethanol extract of Rumex japonicus Houttuyn (RJHE) on anti-obesity activities in 3T3-L1 preadipocytes. In vitro experiments to prevent obesity and improve anti-obesity effects, research using 3T3-L1 preadipocytes is essential, it also distinctly represents morphologic and molecular changes. Our results showed that RJHE decreased the differentiation of 3T3-L1 preadipocytesusing oil red O staining through decreasing the lipid droplet and had no cytotoxicity in 3T3-L1 preadipocytes using WST-1 assay (100 μg/mL and 200 μg/mL). Also, the concentrations of triglycerides(TG) were gradually reduced in 3T3-L1 preadipocytes cells with RJHE (N: 100%, 100 μg/mL: 64.5%, 200 μg/mL: 38.1%) using ELISA assay. And RJHE suppressed the Expression of PPARγ, C/EBPα and Acetyl-CoA Carboxylase (ACC) in a dose-dependent manner by Western blot analysis. Taken together, our results demonstrate that RJHE indicates anti-obesity effects in vitro situation and has potential uses in chemotherapeutic drugs.
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KEYWORD
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Rumex japonicus Houttuyn, 3T3-L1 preadipocytes, anti-obesity
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